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Techniques

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Below is an ongoing list of techniques the lab finds useful for students. If there are interest in other techniques not listed, please feel free to request.

Techniques

Helpful Information

Bioconjugation

Reagents for creating labeled or conjugate molecules via crosslinking, immobilization, modification, biotinylation, and fluorescent labeling of biomolecules

Bioconjugation technical handbook (by Thermo)
Blotting Techniques

Southern and Northern Blotting

Western Blotting

Cell Culture

Cell Culture Basics (by Gibco)

Cell Culture Fundamentals || Video || Invitrogen Handbook

Cell Culture Laboratory Safety || Cell Culture Training 

Preventing Contamination  || Aseptic Techniques

Culture Preparation and Plating

3D Cell Culture vs. 2D Cell Culture

Thawing Cells || Freezing Cells || Passaging Cells

CRIPSR

CRISPR Explained in 2 under 2 mins

CRISPR to Fight Viruses in Human Cells

CRISPR-Cas Biology and Its Application to Infectious Diseases

Culture and Fermentation

Batch & Continuous Culture Basics

Basics of Batch, Fed-Batch, Continuous Culture

Process of Fermentation

Bioethanol Production from Rice Straw

DNA Cloning

The process of making mulitple copies of the same DNA.

Tutorial from Shomu's Biology

UTL Transformation Webinar Slides

The Enzyme Linked Immunosorbent Assay (ELISA)

ELISA is an assay technique used for detecting and quantifying substances like peptides, proteins, antibodies, and hormones.

Step-by-Step from Edvotek Inc.

Webinar Presentation by Dr. Tok

Flow Cytometry

Basic Principles

Multicolor flow cytometry – Understanding the basics (Webinar from Miltenyi Biotec)

Fluorescence Activated Cell Sorting (FACS)

Gel Electrophoresis

Gel electrophoresis is used to separate DNA fragments by size by loading samples from one end of a gel. Electric current is used to pull the DNA through to the other end of the gel.

Overview from A. Agbay

SDS-PAGE Animation 

Sanger DNA Sequencing - Gel Electrophoresis Animation

JoVE - DNA Gel Electrophoresis Video

Organic Solar Cells/Organic Photovoltaics (OPV)

Constructing a Dye Sensitized Solar Cell

Fabrication of dye-sensitized solar cell using blackberry juice

Fabrication of Natural Dye Sensitized Solar Cell (DSSC) based on TiO2 Using Henna And Beetroot Dye Extracts

Stanford Scientists Create All-Carbon Solar Cell

A printable, flexible, organic solar cell (TED Talk by Hannah Bürckstümmer)

Constructing a perovskite solar cell

pH Measurement

Using a pH Meter

How a pH meter works

Top 10 pH Mistakes

Pipette Properly

Pipette is a tool used to measure and transfer liquids from one source to another.

Eppendorf Tutorial

Polymerase chain reaction (PCR)

Polymerase chain reaction (PCR) is a fundamental molecular biology method with many scientific, industrial, and clinical applications.

Overview From A. Agbay

JoVE - The Polymerase Chain Reaction Video

Rotary Evaporation

Basics

Demostration

Streak Plating

Streak plating is used to isolate a pure strain of bacteria from the mixed population.

Video Tutorial
Transformation and Cloning Webinar Recording (presented by A. Agbay)

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